371 Dissecting key features of early-stage atopic dermatitis in pre-clinical models
نویسندگان
چکیده
Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with barrier defects and dysbiosis. The development progression of AD critically depends on the action type 2 immune cells mediators, whose identity chronological appearance in establishment are still elusive. We aim to disclose kinetics cellular contributors responses AD, focusing dynamics interleukin (IL)-4 induction during inflammation pre-clinical models. To mimic impairment, mice were subjected repeated tape stripping (TS) their shaved back skin. Medium containing Staphylococcus aureus (S. aureus) was applied induce Both conditions induced either alone or combined at defined time points. At day six, treated TS S. showed strongest clinical signs inflammation. Using IL-4 reporter mice, we found that combination increased amount IL-4-producing such as basophils, T helper natural killer skin, accompanied by mRNA expression levels IL-13, CCL22. Analyses microbiome WT shift towards predominance Staphylococci one after TS. Thus, impairment likely serves an entry point for Among all relevant cytokines examined, IL-33 most prominent one. On two, detected highest increase protein treatment, indicating additive effect aureus. this no longer visible, since had greater impact production. By contrast, TSLP similarly high groups point, differentially regulated alarmins conditions. show initial phase highly dynamic interplay between damage dysbiosis, contributing differently AD-like These findings will help develop treatment strategies personalized therapy AD.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.09.384